PrimePCR™ Probe Assay: DCAF8, Human

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RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0041419
Assay Design:   exonic
Chromosome Location:   1:160210076-160213763question
Amplicon Length:   68
Splice Variants Targeted:   ENST00000441525 ENST00000506447 ENST00000511820 ENST00000430049 ENST00000325971 ENST00000368073 ENST00000326837 ENST00000368074 ENST00000447377 ENST00000440682 ENST00000407642 ENST00000419626 ENST00000555195 ENST00000556710 ENST00000485079

Gene Information

This gene encodes a WD repeat-containing protein that interacts with the Cul4-Ddb1 E3 ligase macromolecular complex. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq Jul 2009]

Gene Symbol:   DCAF8
Gene Name:   DDB1 and CUL4 associated factor 8
Aliases:   DKFZp781G1096, FLJ35857, H326, MGC117276, MGC118891, MGC99640, WDR42A
RefSeq:   NC_000001.10 NT_004487.19
Ensembl:   ENSG00000132716
Entrez:   50717
UniGene:   Hs.632447
Chromosome Mapping:   1q22-q23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.730000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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