PrimePCR™ Probe Assay: ADORA2A, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: ADORA2A, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ADORA2A, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0041414
Assay Design:   exonic
Chromosome Location:   22:24829353-24829479question
Amplicon Length:   97
Splice Variants Targeted:   ENST00000545677 ENST00000454808 ENST00000543955 ENST00000498926 ENST00000317555 ENST00000590546 ENST00000217254 ENST00000424232 ENST00000444262 ENST00000337539 ENST00000436735 ENST00000439591 ENST00000336332 ENST00000380752 ENST00000331105 ENST00000393409 ENST00000251772 ENST00000586927 ENST00000252595 ENST00000403084 ENST00000356357 ENST00000309285 ENST00000262027 ENST00000315473 ENST00000400186 ENST00000602723 ENST00000520002 ENST00000602557 ENST00000537824 ENST00000542608 ENST00000268489 ENST

Gene Information

This gene encodes a protein which is one of several receptor subtypes for adenosine. The activity of the encoded protein a G-protein coupled receptor family member is mediated by G proteins which activate adenylyl cyclase. The encoded protein is abundant in basal ganglia vasculature and platelets and it is a major target of caffeine. [provided by RefSeq Jul 2008]

Gene Symbol:   ADORA2A
Gene Name:   adenosine A2a receptor
Aliases:   ADORA2, RDC8, hA2aR
RefSeq:   NC_000022.10 NT_011520.12
Ensembl:   ENSG00000128271
Entrez:   135
Chromosome Mapping:   22q11.23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 36.430000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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