PrimePCR™ Probe Assay: TUBA1B, Human

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RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0041407
Assay Design:   exonic
Chromosome Location:   12:49523283-49523379question
Amplicon Length:   67
Splice Variants Targeted:   ENST00000400113 ENST00000336023 ENST00000550367 ENST00000549870 ENST00000547476 ENST00000552984 ENST00000301071 ENST00000295766 ENST00000550767 ENST00000547939 ENST00000546918 ENST00000552924 ENST00000541364 ENST00000301072 ENST00000549183 ENST00000312988 ENST00000321253

Gene Information

Description Not Available

Gene Symbol:   TUBA1B
Gene Name:   tubulin, alpha 1b
Aliases:   K-ALPHA-1
RefSeq:   NC_000012.11 NT_029419.12
Ensembl:   ENSG00000123416
Entrez:   10376
UniGene:   Hs.524390
Chromosome Mapping:   12q13.12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 36.040000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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