PrimePCR™ Probe Assay: BTN3A3, Human

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Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0041398
Assay Design:   exonic
Chromosome Location:   6:26444261-26444374question
Amplicon Length:   84
Splice Variants Targeted:   ENST00000380250 ENST00000532865 ENST00000530653 ENST00000527417 ENST00000527422 ENST00000356386 ENST00000377708 ENST00000396948 ENST00000396934 ENST00000508906 ENST00000325239 ENST00000413659 ENST00000476549 ENST00000289361 ENST00000450085 ENST00000425234 ENST00000506698 ENST00000414912 ENST00000494393 ENST00000482451 ENST00000244519 ENST00000471353 ENST00000361232 ENST00000487627 ENST00000496719 ENST00000490254 ENST00000487272 ENST00000339789 ENST00000408931

Gene Information

The butyrophilin (BTN) genes are a group of major histocompatibility complex (MHC)-associated genes that encode type I membrane proteins with 2 extracellular immunoglobulin (Ig) domains and an intracellular B30.2 (PRYSPRY) domain. Three subfamilies of human BTN genes are located in the MHC class I region: the single-copy BTN1A1 gene (MIM 601610) and the BTN2 (e.g. BTN2A1; MIM 613590) and BTN3 (e.g. BNT3A3) genes which have undergone tandem duplication resulting in 3 copies of each (summary by Smith et al. 2010 [PubMed 20208008]).[supplied by OMIM Nov 2010]

Gene Symbol:   BTN3A3
Gene Name:   butyrophilin, subfamily 3, member A3
Aliases:   BTF3
RefSeq:   NC_000006.11 NT_007592.15
Ensembl:   ENSG00000111801
Entrez:   10384
UniGene:   Hs.167741
Chromosome Mapping:   6p21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.995600
y-intercept 35.620000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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