PrimePCR™ Probe Assay: CYP2D6, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: CYP2D6, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: CYP2D6, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0041377
Assay Design:   exonic
Chromosome Location:   22:42526633-42526848question
Amplicon Length:   186
Splice Variants Targeted:   ENST00000315684 ENST00000393567 ENST00000360608 ENST00000359033 ENST00000389970 ENST00000332408 ENST00000396535 ENST00000537958 ENST00000427980 ENST00000393853 ENST00000240335 ENST00000373555 ENST00000254605 ENST00000393597 ENST00000311131 ENST00000307142 ENST00000504178 ENST00000261177 ENST00000398786 ENST00000545760 ENST00000441508 ENST00000372146

Gene Information

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol steroids and other lipids. This protein localizes to the endoplasmic reticulum and is known to metabolize as many as 20% of commonly prescribed drugs. Its substrates include debrisoquine an adrenergic-blocking drug; sparteine and propafenone both anti-arrythmic drugs; and amitryptiline an anti-depressant. The gene is highly polymorphic in the population; certain alleles result in the poor metabolizer phenotype characterized by a decreased ability to metabolize the enzyme's substrates. The gene is located near two cytochrome P450 pseudogenes on chromosome 22q13.1. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   CYP2D6
Gene Name:   cytochrome P450, family 2, subfamily D, polypeptide 6
Aliases:   CPD6, CYP2D, CYP2D7AP, CYP2D7BP, CYP2D7P2, CYP2D8P2, CYP2DL1, CYPIID6, MGC120389, MGC120390, P450-DB1, P450C2D, P450DB1
RefSeq:   NC_000022.10 NG_003180.2 NG_008376.1 NT_011520.12
Ensembl:   ENSG00000100197
Entrez:   1565
Chromosome Mapping:   22q13.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998200
y-intercept 35.840000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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