PrimePCR™ Probe Assay: ALG9, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: ALG9, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ALG9, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0041371
Assay Design:   exonic
Chromosome Location:   11:111708194-111708294question
Amplicon Length:   71
Splice Variants Targeted:   ENST00000571337 ENST00000585430 ENST00000587827 ENST00000592795 ENST00000586097 ENST00000585398 ENST00000379565 ENST00000544447 ENST00000379548 ENST00000358531 ENST00000371027 ENST00000445265 ENST00000323668 ENST00000451292 ENST00000377797 ENST00000245503 ENST00000397183 ENST00000373247 ENST00000373225 ENST00000373245 ENST00000530275 ENST00000586985 ENST00000532425 ENST00000531154 ENST00000398006 ENST00000328459 ENST00000357056 ENST00000374316 ENST00000230895 ENST00000398148

Gene Information

This gene encodes an alpha-12-mannosyltransferase enzyme that functions in lipid-linked oligosaccharide assembly. Mutations in this gene result in congenital disorder of glycosylation type Il. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Dec 2008]

Gene Symbol:   ALG9
Gene Name:   asparagine-linked glycosylation 9, alpha-1,2-mannosyltransferase homolog (S. cerevisiae)
Aliases:   CDG1L, DIBD1, DKFZp586M2420, FLJ21845, LOH11CR1J
RefSeq:   NC_000011.9 NT_033899.8 NG_009210.1
Ensembl:   ENSG00000086848
Entrez:   79796
Chromosome Mapping:   11q23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.880000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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