PrimePCR™ Probe Assay: WBSCR22, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: WBSCR22, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: WBSCR22, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0041367
Assay Design:   exonic
Chromosome Location:   7:73112162-73112254question
Amplicon Length:   63
Splice Variants Targeted:   ENST00000265758 ENST00000423497 ENST00000442099 ENST00000453316 ENST00000590071 ENST00000574150 ENST00000572912 ENST00000570412 ENST00000573915

Gene Information

This gene encodes a protein containing a nuclear localization signal and an S-adenosyl-L-methionine binding motif typical of methyltransferases suggesting that the encoded protein may act on DNA methylation. This gene is deleted in Williams syndrome a multisystem developmental disorder caused by the deletion of contiguous genes at 7q11.23. Alternatively spliced transcript variants have been found. [provided by RefSeq Feb 2011]

Gene Symbol:   WBSCR22
Gene Name:   Williams Beuren syndrome chromosome region 22
Aliases:   FLJ44236, HASJ4442, HUSSY-3, MGC19709, MGC2022, MGC5140, PP3381, WBMT
RefSeq:   NG_013360.1 NC_000007.13 NT_007933.15
Ensembl:   ENSG00000071462
Entrez:   114049
Chromosome Mapping:   -

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 35.620000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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