This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.
PrimePCR™ PreAmp for Probe Assay: DCTN3, Human
PrimePCR™ Template for Probe Assay: DCTN3, Human
This gene encodes the smallest subunit of dynactin a macromolecular complex consisting of 10 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. It is involved in a diverse array of cellular functions including ER-to-Golgi transport the centripetal movement of lysosomes and endosomes spindle formation cytokinesis chromosome movement nuclear positioning and axonogenesis. This subunit like most other dynactin subunits exists only as a part of the dynactin complex. It is primarily an alpha-helical protein with very little coiled coil and binds directly to the largest subunit (p150) of dynactin. Alternative splicing of this gene generates 2 transcript variants. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.