PrimePCR™ Probe Assay: TINAGL1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0041305
Assay Design:   exonic
Chromosome Location:   1:32050488-32050582question
Amplicon Length:   65
Splice Variants Targeted:   ENST00000271064 ENST00000457433 ENST00000537531

Gene Information

The protein encoded by this gene is similar in sequence to tubulointerstitial nephritis antigen a secreted glycoprotein that is recognized by antibodies in some types of immune-related tubulointerstitial nephritis. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Feb 2011]

Gene Symbol:   TINAGL1
Gene Name:   tubulointerstitial nephritis antigen-like 1
Aliases:   ARG1, LCN7, LIECG3, TINAGRP
RefSeq:   NC_000001.10 NG_028164.1 NT_032977.9
Ensembl:   ENSG00000142910
Entrez:   64129
UniGene:   Hs.199368
Chromosome Mapping:   1p35.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.770000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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