PrimePCR™ Probe Assay: RP11-653A5.2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: RP11-653A5.2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: RP11-653A5.2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0041194
Assay Design:   exonic
Chromosome Location:   1:74665348-74665456question
Amplicon Length:   79
Splice Variants Targeted:   ENST00000467578 ENST00000524915 ENST00000482102 ENST00000370898 ENST00000370894 ENST00000534056 ENST00000472069 ENST00000370899 ENST00000370895 ENST00000534632 ENST00000557284 ENST00000370893 ENST00000370891

Gene Information

Description Not Available

Gene Symbol:   RP11-653A5.2
Gene Name:   FPGT-TNNI3K fusion protein isoform a
Aliases:   DKFZp686C06106, MGC33828
RefSeq:   Not Available
Ensembl:   ENSG00000259030
Entrez:   100526835
Chromosome Mapping:   1p31.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 36.530000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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