PrimePCR™ Probe Assay: ATP6V1G2-DDX39B, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: ATP6V1G2-DDX39B, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ATP6V1G2-DDX39B, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0041180
Assay Design:   exonic
Chromosome Location:   6:31513886-31514258question
Amplicon Length:   74
Splice Variants Targeted:   ENST00000539050 ENST00000535898 ENST00000371270 ENST00000233946 ENST00000410023 ENST00000409589 ENST00000393409 ENST00000251772 ENST00000444088 ENST00000445218 ENST00000354495 ENST00000475917 ENST00000449748 ENST00000331493 ENST00000517484 ENST00000303892 ENST00000483251 ENST00000415099 ENST00000414440 ENST00000602465 ENST00000265865 ENST00000388768 ENST00000456666 ENST00000454889 ENST00000552135 ENST00000454370 ENST00000412106 ENST00000441959 ENST00000374518 ENST00000383508 ENST00000383503 ENST

Gene Information

This locus represents naturally occurring read-through transcription between the neighboring ATP6V1G2 (ATPase H+ transporting lysosomal 13kDa V1 subunit G2) and DDX39B (DEAD box polypeptide 39B) genes located in the major histocompatibility complex class III region of chromosome 6. The read-through transcript and is a candidate for nonsense-mediated mRNA decay (NMD) and is thus unlikely to produce a protein product. [provided by RefSeq Feb 2011]

Gene Symbol:   ATP6V1G2-DDX39B
Gene Name:   ATP6V1G2-DDX39B
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSG00000254870
Entrez:   100532737
Chromosome Mapping:   6p

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 36.300000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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