PrimePCR™ Probe Assay: PRR5-ARHGAP8, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: PRR5-ARHGAP8, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: PRR5-ARHGAP8, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0041167
Assay Design:   exonic
Chromosome Location:   22:45258366-45258458question
Amplicon Length:   63
Splice Variants Targeted:   ENST00000340908 ENST00000530271 ENST00000537687 ENST00000315939 ENST00000535572 ENST00000342427 ENST00000375200 ENST00000400358 ENST00000400359 ENST00000361473 ENST00000352766 ENST00000515632 ENST00000389774 ENST00000336963 ENST00000356099 ENST00000517296 ENST00000389773 ENST00000340181 ENST00000373737 ENST00000373738 ENST00000538992 ENST00000283441 ENST00000424784 ENST00000246949 ENST00000448662 ENST00000373094 ENST00000373097 ENST00000361655 ENST00000406881 ENST00000504220 ENST00000373106 ENST

Gene Information

Description Not Available

Gene Symbol:   PRR5-ARHGAP8
Gene Name:   PRR5-ARHGAP8 readthrough
Aliases:   Not Available
RefSeq:   NC_000022.10 NT_011520.12
Ensembl:   ENSG00000248405
Entrez:   553158
Chromosome Mapping:   22q13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 36.620000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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