PrimePCR™ Probe Assay: WASH4P, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: WASH4P, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: WASH4P, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0041142
Assay Design:   exonic
Chromosome Location:   16:64220-64354question
Amplicon Length:   105
Splice Variants Targeted:   ENST00000334554 ENST00000320602 ENST00000405930 ENST00000326592 ENST00000359512 ENST00000285718 ENST00000351293 ENST00000322635 ENST00000423381 ENST00000322680 ENST00000394762 ENST00000370377 ENST00000355149 ENST00000437787 ENST00000296786 ENST00000597984 ENST00000601765 ENST00000600347

Gene Information

Description Not Available

Gene Symbol:   WASH4P
Gene Name:   WAS protein family homolog 4 pseudogene
Aliases:   CXYorf1P, FAM39CP, FLJ31670
RefSeq:   NC_000016.9 NG_003159.3 NT_010393.16
Ensembl:   ENSG00000234769
Entrez:   374677
Chromosome Mapping:   16p13.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998200
y-intercept 35.840000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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