PrimePCR™ Probe Assay: CERS1, Human

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RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0041123
Assay Design:   exonic
Chromosome Location:   19:18990050-18990142question
Amplicon Length:   63
Splice Variants Targeted:   ENST00000237536 ENST00000357779 ENST00000456801 ENST00000397795 ENST00000256552 ENST00000558768 ENST00000558445 ENST00000559177 ENST00000542188 ENST00000318351 ENST00000416617 ENST00000238823 ENST00000403368 ENST00000441530 ENST00000382348 ENST00000333063 ENST00000559624 ENST00000263791 ENST00000382727 ENST00000520200 ENST00000261800 ENST00000360529 ENST00000461742 ENST00000429504 ENST00000542296 ENST00000596048 ENST00000427170 ENST00000247005 ENST00000249647 ENST00000310441 ENST00000369984 ENST

Gene Information

Description Not Available

Gene Symbol:   CERS1
Gene Name:   ceramide synthase 1
Aliases:   CerS1, LAG1, MGC90349, UOG1
RefSeq:   NC_000019.9 NG_003110.1 NG_012070.1 NT_011295.11
Ensembl:   ENSG00000223802
Entrez:   10715
UniGene:   Not Available
Chromosome Mapping:   19p12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.720000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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