PrimePCR™ Probe Assay: CKMT1A, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: CKMT1A, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: CKMT1A, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0041121
Assay Design:   exonic
Chromosome Location:   15:43986205-43986300question
Amplicon Length:   66
Splice Variants Targeted:   ENST00000411594 ENST00000230901 ENST00000418329 ENST00000454473 ENST00000441656 ENST00000538936 ENST00000331285 ENST00000538721 ENST00000571105 ENST00000570391 ENST00000572995 ENST00000570388 ENST00000576343 ENST00000399848 ENST00000359446 ENST00000361205 ENST00000399333 ENST00000373347 ENST00000235180 ENST00000240328 ENST00000453782 ENST00000437924 ENST00000449946 ENST00000417289 ENST00000441322 ENST00000453733 ENST00000300283 ENST00000450086 ENST00000413657 ENST00000299687 ENST00000318080 ENST

Gene Information

Mitochondrial creatine (MtCK) kinase is responsible for the transfer of high energy phosphate from mitochondria to the cytosolic carrier creatine. It belongs to the creatine kinase isoenzyme family. It exists as two isoenzymes sarcomeric MtCK and ubiquitous MtCK encoded by separate genes. Mitochondrial creatine kinase occurs in two different oligomeric forms: dimers and octamers in contrast to the exclusively dimeric cytosolic creatine kinase isoenzymes. Many malignant cancers with poor prognosis have shown overexpression of ubiquitous mitochondrial creatine kinase; this may be related to high energy turnover and failure to eliminate cancer cells via apoptosis. Ubiquitous mitochondrial creatine kinase has 80% homology with the coding exons of sarcomeric mitochondrial creatine kinase. Two genes located near each other on chromosome 15 have been identified which encode identical mitochondrial creatine kinase proteins. [provided by RefSeq Jul 2008]

Gene Symbol:   CKMT1A
Gene Name:   creatine kinase, mitochondrial 1A
Aliases:   CKMT1, CKMT1B, FLJ50967, UMTCK
RefSeq:   NC_000015.9 NG_005156.1 NT_010194.17
Ensembl:   ENSG00000223572
Entrez:   548596
Chromosome Mapping:   15q15

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.600000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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