PrimePCR™ Probe Assay: VPS52, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: VPS52, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: VPS52, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0041120
Assay Design:   exonic
Chromosome Location:   6:33236804-33236935question
Amplicon Length:   102
Splice Variants Targeted:   ENST00000448042 ENST00000320631 ENST00000383210 ENST00000226091 ENST00000328656 ENST00000398930 ENST00000398934 ENST00000319080 ENST00000538698 ENST00000539039 ENST00000352035 ENST00000590151 ENST00000537919 ENST00000393896 ENST00000353196 ENST00000475390 ENST00000241274 ENST00000428608 ENST00000330342 ENST00000539925 ENST00000228918 ENST00000536876 ENST00000543190 ENST00000377633 ENST00000234198 ENST00000263800 ENST00000482399 ENST00000445902 ENST00000436044 ENST00000441058 ENST00000369905 ENST

Gene Information

This gene encodes a protein that is similar to the yeast suppressor of actin mutations 2 gene. The yeast protein forms a subunit of the tetrameric Golgi-associated retrograde protein complex that is involved in vesicle trafficking from from both early and late endosomes back to the trans-Golgi network. This gene is located on chromosome 6 in a head-to-head orientation with the gene encoding ribosomal protein S18. [provided by RefSeq Jul 2008]

Gene Symbol:   VPS52
Gene Name:   vacuolar protein sorting 52 homolog (S. cerevisiae)
Aliases:   ARE1, DKFZp547I194, RP5-1033B10, SAC2, SACM2L, dJ1033B10.5
RefSeq:   NC_000006.11 NT_007592.15 NT_113891.2 NT_167245.1 NT_167246.1 NT_167247.1 NT_167248.1 NT_167249.1
Ensembl:   ENSG00000223501
Entrez:   6293
Chromosome Mapping:   6p21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.710000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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