PrimePCR™ Probe Assay: ZNF630, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: ZNF630, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ZNF630, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0041117
Assay Design:   exonic
Chromosome Location:   X:47926348-47930438question
Amplicon Length:   78
Splice Variants Targeted:   ENST00000409324 ENST00000276054 ENST00000428686 ENST00000421903 ENST00000442455 ENST00000600445 ENST00000600631 ENST00000599149 ENST00000597631 ENST00000595420

Gene Information

This gene encodes a zinc finger protein with an N-terminal Kruppel-associated box-containing (KRAB) domain and 13 Kruppel-type C2H2 zinc finger domains. Like other zinc finger proteins this gene may function as a transcription factor. This gene resides on an area of chromosome X that has been implicated in nonsyndromic X-linked mental retardation. [provided by RefSeq Jul 2010]

Gene Symbol:   ZNF630
Gene Name:   zinc finger protein 630
Aliases:   FLJ20573, FLJ97927, FLJ98691, MGC138344, dJ54B20.2
RefSeq:   NC_000023.10 NG_022889.1 NT_079573.4
Ensembl:   ENSG00000221994
Entrez:   57232
Chromosome Mapping:   Xp11.3-p11.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997100
y-intercept 35.260000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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