PrimePCR™ Probe Assay: MAGEA3, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: MAGEA3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: MAGEA3, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0041112
Assay Design:   exonic
Chromosome Location:   X:151935718-151935861question
Amplicon Length:   114
Splice Variants Targeted:   ENST00000306773 ENST00000296452 ENST00000262895 ENST00000593562 ENST00000301218 ENST00000381222 ENST00000381218 ENST00000381223 ENST00000375774 ENST00000449131 ENST00000582679 ENST00000396801 ENST00000361959 ENST00000355772 ENST00000396799 ENST00000417772 ENST00000319770 ENST00000396795 ENST00000299178 ENST00000402708 ENST00000035307 ENST00000495645 ENST00000570156 ENST00000441106 ENST00000422127 ENST00000366707 ENST00000329342 ENST00000412733 ENST00000457643 ENST00000370293 ENST00000423993 ENST

Gene Information

This gene is a member of the MAGEA gene family. The members of this family encode proteins with 50 to 80% sequence identity to each other. The promoters and first exons of the MAGEA genes show considerable variability suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are clustered at chromosomal location Xq28. They have been implicated in some hereditary disorders such as dyskeratosis congenita. [provided by RefSeq Jul 2008]

Gene Symbol:   MAGEA3
Gene Name:   melanoma antigen family A, 3
Aliases:   CT1.3, HIP8, HYPD, MAGE3, MAGEA6, MGC14613
RefSeq:   NC_000023.10 NG_013244.1 NT_167198.1
Ensembl:   ENSG00000221867
Entrez:   4102
Chromosome Mapping:   Xq28

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 35.440000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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