PrimePCR™ Probe Assay: TP53TG3B, Human

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RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0041070
Assay Design:   exonic
Chromosome Location:   16:33205754-33205845question
Amplicon Length:   62
Splice Variants Targeted:   ENST00000399012 ENST00000381657 ENST00000369784 ENST00000216962 ENST00000398664 ENST00000569631 ENST00000354614 ENST00000398682 ENST00000569420 ENST00000398680 ENST00000561509 ENST00000360260 ENST00000398667 ENST00000398666 ENST00000341305 ENST00000569741 ENST00000380147 ENST00000355235 ENST00000356834 ENST00000354329 ENST00000527372 ENST00000531253 ENST00000533112

Gene Information

Description Not Available

Gene Symbol:   TP53TG3B
Gene Name:   TP53 target 3B
Aliases:   MGC119888, TP53TG3, TP53TG3A, TP53TG3C, TP53TG3D
RefSeq:   NC_000016.9 NT_010393.16
Ensembl:   ENSG00000205457
Entrez:   729355
UniGene:   Hs.592038
Chromosome Mapping:   16p11.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 35.440000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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