PrimePCR™ Probe Assay: SARNP, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: SARNP, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: SARNP, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0041068
Assay Design:   exonic
Chromosome Location:   12:56151158-56151257question
Amplicon Length:   70
Splice Variants Targeted:   ENST00000253354 ENST00000336133 ENST00000444631 ENST00000546837 ENST00000381947 ENST00000314399 ENST00000402202 ENST00000572540 ENST00000571432 ENST00000573306

Gene Information

This gene encodes a protein that is upregulated in response to various cytokines. The encoded protein may play a role in cell cycle progression. A translocation between this gene and the myeloid/lymphoid leukemia gene resulting in expression of a chimeric protein has been associated with acute myelomonocytic leukemia. Pseudogenes exist on chromosomes 7 and 8. Alternatively spliced transcript variants have been described. [provided by RefSeq Feb 2009]

Gene Symbol:   SARNP
Gene Name:   SAP domain containing ribonucleoprotein
Aliases:   CIP29, HCC1, HSPC316, MGC14726, THO1
RefSeq:   NC_000012.11 NT_029419.12
Ensembl:   ENSG00000205323
Entrez:   84324
Chromosome Mapping:   12q13.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.988600
y-intercept 34.070000
Efficiency 110

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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