PrimePCR™ Probe Assay: PSORS1C1, Human

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Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0041057
Assay Design:   exonic
Chromosome Location:   6:31107591-31107732question
Amplicon Length:   112
Splice Variants Targeted:   ENST00000441092 ENST00000382112 ENST00000382111 ENST00000400246 ENST00000382105 ENST00000373509 ENST00000433334 ENST00000420214 ENST00000259881 ENST00000547221 ENST00000481450 ENST00000448455 ENST00000342644 ENST00000372027 ENST00000310355 ENST00000270530 ENST00000376289 ENST00000549149 ENST00000344204 ENST00000509967 ENST00000513206 ENST00000537187

Gene Information

This gene is one of several genes thought to confer susceptibility to psoriasis and systemic sclerosis located on chromosome 6 near the major histocompatibility complex (MHC) class I region. [provided by RefSeq Sep 2011]

Gene Symbol:   PSORS1C1
Gene Name:   psoriasis susceptibility 1 candidate 1
Aliases:   C6orf16, MGC132530, SEEK1
RefSeq:   NC_000006.11 NG_012192.1 NG_021348.1 NT_007592.15 NT_113891.2 NT_167245.1 NT_167246.1 NT_167247.1 NT_167248.1
Ensembl:   ENSG00000204540
Entrez:   170679
UniGene:   Hs.310958
Chromosome Mapping:   6p21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.610000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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