PrimePCR™ Probe Assay: PTPN20A, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: PTPN20A, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: PTPN20A, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0041046
Assay Design:   exonic
Chromosome Location:   10:46587344-46587483question
Amplicon Length:   110
Splice Variants Targeted:   ENST00000331650 ENST00000374240 ENST00000374233 ENST00000507417 ENST00000514592 ENST00000515532 ENST00000514491 ENST00000509230 ENST00000509631 ENST00000510840 ENST00000512924 ENST00000509753 ENST00000502266 ENST00000417914 ENST00000395660 ENST00000374235 ENST00000395661 ENST00000395668 ENST00000395662 ENST00000395721 ENST00000374346 ENST00000508602 ENST00000374339 ENST00000509900 ENST00000506080 ENST00000513266 ENST00000502705 ENST00000505814 ENST00000509774 ENST00000511769 ENST00000509599 ENST

Gene Information

The product of this gene belongs to the family of classical tyrosine-specific protein tyrosine phosphatases. Many protein tyrosine phosphatases have been shown to regulate fundamental cellular processes and several are mutated in human diseases. Chromosome 10q contains a segmental duplication resulting in multiple copies of the protein tyrosine phosphatase non-receptor type 20 gene. The two nearly identical copies are designated as PTPN20A and PTPN20B. A third copy is only partially duplicated and contains a pseudogene designated as PTPN20C. This gene encodes the more centromeric copy PTPN20A. Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq Jul 2008]

Gene Symbol:   PTPN20A
Gene Name:   protein tyrosine phosphatase, non-receptor type 20A
Aliases:   CT126, MGC142033, PTPN20B, bA142I17.1, hPTPN20
RefSeq:   NC_000010.10 NG_005598.1 NT_031847.8
Ensembl:   ENSG00000204179
Entrez:   653129
Chromosome Mapping:   10q11.22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998000
y-intercept 35.260000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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