PrimePCR™ Probe Assay: AGAP10, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: AGAP10, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: AGAP10, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0041045
Assay Design:   exonic
Chromosome Location:   10:47193343-47193468question
Amplicon Length:   96
Splice Variants Targeted:   ENST00000252322 ENST00000456984 ENST00000453919 ENST00000521776 ENST00000602930 ENST00000425119 ENST00000479304 ENST00000448048 ENST00000374095 ENST00000412531 ENST00000374056 ENST00000301067 ENST00000452145 ENST00000413193 ENST00000355232 ENST00000443782 ENST00000374094 ENST00000379509 ENST00000309052 ENST00000540623 ENST00000394422 ENST00000425117 ENST00000371051 ENST00000371042 ENST00000342551 ENST00000311652 ENST00000584334 ENST00000581330

Gene Information

Description Not Available

Gene Symbol:   AGAP10
Gene Name:   ArfGAP with GTPase domain, ankyrin repeat and PH domain 10
Aliases:   CTGLF7, bA144G6.2
RefSeq:   NC_000010.10 NT_031847.8
Ensembl:   ENSG00000204172
Entrez:   728127
Chromosome Mapping:   10q11.22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998000
y-intercept 36.270000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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