PrimePCR™ Probe Assay: IL28B, Human

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Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0041006
Assay Design:   exonic
Chromosome Location:   19:39735523-39735646question
Amplicon Length:   94
Splice Variants Targeted:   ENST00000422132 ENST00000423807 ENST00000419060 ENST00000262715 ENST00000556935 ENST00000545983 ENST00000223095 ENST00000445463 ENST00000405694 ENST00000263776 ENST00000413851

Gene Information

This gene encodes a cytokine distantly related to type I interferons and the IL-10 family. This gene interleukin 28A (IL28A) and interleukin 29 (IL29) are three closely related cytokine genes that form a cytokine gene cluster on a chromosomal region mapped to 19q13. Expression of the cytokines encoded by the three genes can be induced by viral infection. All three cytokines have been shown to interact with a heterodimeric class II cytokine receptor that consists of interleukin 10 receptor beta (IL10RB) and interleukin 28 receptor alpha (IL28RA). [provided by RefSeq Jul 2008]

Gene Symbol:   IL28B
Gene Name:   interleukin 28B (interferon, lambda 3)
Aliases:   IFNL3, IL-28B, IL28C
RefSeq:   NC_000019.9 NT_011109.16
Ensembl:   ENSG00000197110
Entrez:   282617
UniGene:   Hs.406744
Chromosome Mapping:   19q13.13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.670000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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