PrimePCR™ Probe Assay: FAM163B, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: FAM163B, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: FAM163B, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0041003
Assay Design:   exonic
Chromosome Location:   9:136444424-136444514question
Amplicon Length:   61
Splice Variants Targeted:   ENST00000400676 ENST00000537152 ENST00000327787 ENST00000564535 ENST00000568089 ENST00000534366 ENST00000528919 ENST00000398656 ENST00000326134 ENST00000367467 ENST00000261388 ENST00000572599 ENST00000398665 ENST00000440640 ENST00000240328 ENST00000496132 ENST00000356873 ENST00000357164 ENST00000301732 ENST00000382381 ENST00000333850 ENST00000332539 ENST00000311601 ENST00000483233 ENST00000315877 ENST00000035307 ENST00000495645

Gene Information

Description Not Available

Gene Symbol:   FAM163B
Gene Name:   family with sequence similarity 163, member B
Aliases:   C9orf166
RefSeq:   NC_000009.11 NT_035014.4
Ensembl:   ENSG00000196990
Entrez:   642968
Chromosome Mapping:   9q34.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.840000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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