PrimePCR™ Probe Assay: ZNF124, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: ZNF124, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ZNF124, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040994
Assay Design:   exonic
Chromosome Location:   1:247335151-247335265question
Amplicon Length:   85
Splice Variants Targeted:   ENST00000253686 ENST00000444840 ENST00000378115 ENST00000543357 ENST00000056217 ENST00000372406 ENST00000347136 ENST00000372410 ENST00000593756 ENST00000472531 ENST00000491356 ENST00000340684 ENST00000543802 ENST00000258381 ENST00000358662 ENST00000392048 ENST00000258382 ENST00000540870 ENST00000338556 ENST00000594046 ENST00000594105 ENST00000595693

Gene Information

This gene encodes a member of the Kruppel-type (C2H2) zinc finger protein family. It has a KRAB-A box at its amino terminus and seven consecutively repeated zinc finger motifs at its carboxy terminus. Expression of this gene is increased after vascular endothelial growth factor (VEGF) stimulation in human leukemia cell lines and its expression inhibits apoptotic cell death induced by irradiation or exposure to etoposide. These attributes suggest that this protein may act as a transcription factor. Alternative splicing results in multiple transcript variants encoding distinct proteins. Additional transcript variants have been described for this gene but their biological validity has not been confirmed. This gene has a pseudogene on chromosome 14. [provided by RefSeq Aug 2011]

Gene Symbol:   ZNF124
Gene Name:   zinc finger protein 124
Aliases:   HZF-16, HZF16, MGC117046
RefSeq:   NC_000001.10 NT_167186.1
Ensembl:   ENSG00000196418
Entrez:   7678
Chromosome Mapping:   1q44

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998500
y-intercept 34.880000
Efficiency 105

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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