PrimePCR™ Probe Assay: DEFB106B, Human

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RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040975
Assay Design:   exonic
Chromosome Location:   8:7340136-7340248question
Amplicon Length:   83
Splice Variants Targeted:   ENST00000005386 ENST00000432584 ENST00000380650 ENST00000335186 ENST00000409202 ENST00000409030 ENST00000409220 ENST00000497259 ENST00000479844 ENST00000295381 ENST00000324685 ENST00000563683 ENST00000357890 ENST00000382004 ENST00000335479 ENST00000354366 ENST00000357904 ENST00000304623 ENST00000511377 ENST00000503622 ENST00000359640 ENST00000458100

Gene Information

Defensins form a family of microbicidal and cytotoxic peptides made by neutrophils. Defensins are short processed peptide molecules that are classified by structure into three groups: alpha-defensins beta-defensins and theta-defensins. All beta-defensin genes are densely clustered in four to five syntenic chromosomal regions. Chromosome 8p23 contains at least two copies of the duplicated beta-defensin cluster. This duplication results in two identical copies of defensin beta 106 DEFB106A and DEFB106B in head-to-head orientation. This gene DEFB106B represents the more telomeric copy. [provided by RefSeq Jul 2008]

Gene Symbol:   DEFB106B
Gene Name:   defensin, beta 106B
Aliases:   Not Available
RefSeq:   NC_000008.10 NG_005589.1 NT_023736.17
Ensembl:   ENSG00000187082
Entrez:   503841
UniGene:   Hs.511958
Chromosome Mapping:   8p23.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 35.880000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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