PrimePCR™ Probe Assay: DEFB105B, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: DEFB105B, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: DEFB105B, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040970
Assay Design:   exonic
Chromosome Location:   8:7346949-7347055question
Amplicon Length:   77
Splice Variants Targeted:   ENST00000334773 ENST00000335510

Gene Information

Defensins form a family of microbicidal and cytotoxic peptides made by neutrophils. Defensins are short processed peptide molecules that are classified by structure into three groups: alpha-defensins beta-defensins and theta-defensins. All beta-defensin genes are densely clustered in four to five syntenic chromosomal regions. Chromosome 8p23 contains at least two copies of the duplicated beta-defensin cluster. This duplication results in two identical copies of defensin beta 105 DEFB105A and DEFB105B in tail-to-tail orientation. This gene DEFB105B represents the more telomeric copy. [provided by RefSeq Jul 2008]

Gene Symbol:   DEFB105B
Gene Name:   defensin, beta 105B
Aliases:   DEFB105A
RefSeq:   NC_000008.10 NG_005589.1 NT_023736.17
Ensembl:   ENSG00000186599
Entrez:   504180
Chromosome Mapping:   8p23.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 36.170000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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