PrimePCR™ Probe Assay: FCAR, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: FCAR, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: FCAR, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040969
Assay Design:   exonic
Chromosome Location:   19:55396776-55396874question
Amplicon Length:   69
Splice Variants Targeted:   ENST00000392782 ENST00000392783 ENST00000541068 ENST00000355831 ENST00000343439 ENST00000391726 ENST00000355524 ENST00000391725 ENST00000345937 ENST00000359272 ENST00000391723 ENST00000391724 ENST00000433231 ENST00000571891 ENST00000572552 ENST00000576568 ENST00000575913 ENST00000575866 ENST00000576509 ENST00000573084 ENST00000572443 ENST00000574618 ENST00000571746 ENST00000576265 ENST00000573433 ENST00000574058 ENST00000575074 ENST00000573273 ENST00000574738

Gene Information

This gene is a member of the immunoglobulin gene superfamily and encodes a receptor for the Fc region of IgA. The receptor is a transmembrane glycoprotein present on the surface of myeloid lineage cells such as neutrophils monocytes macrophages and eosinophils where it mediates immunologic responses to pathogens. It interacts with IgA-opsonized targets and triggers several immunologic defense processes including phagocytosis antibody-dependent cell-mediated cytotoxicity and stimulation of the release of inflammatory mediators. Multiple alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   FCAR
Gene Name:   Fc fragment of IgA, receptor for
Aliases:   CD89
RefSeq:   NC_000019.9 NT_011109.16
Ensembl:   ENSG00000186431
Entrez:   2204
Chromosome Mapping:   19q13.42

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.390000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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