PrimePCR™ Probe Assay: TP53TG3, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: TP53TG3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: TP53TG3, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040953
Assay Design:   exonic
Chromosome Location:   16:32687204-32687351question
Amplicon Length:   118
Splice Variants Targeted:   ENST00000370640 ENST00000280098 ENST00000372231 ENST00000438331 ENST00000371739 ENST00000344576 ENST00000243662 ENST00000526372 ENST00000398664 ENST00000569631 ENST00000354614 ENST00000398682 ENST00000569420 ENST00000398680 ENST00000561509 ENST00000360260 ENST00000398667 ENST00000398666 ENST00000341305 ENST00000569741 ENST00000380147 ENST00000397910 ENST00000369005 ENST00000515273 ENST00000515603 ENST00000334433 ENST00000453444 ENST00000581449

Gene Information

Description Not Available

Gene Symbol:   TP53TG3
Gene Name:   TP53 target 3
Aliases:   P53TG3, TP53TG3A, TP53TG3B, TP53TG3C, TP53TG3D
RefSeq:   NC_000016.9 NT_010393.16
Ensembl:   ENSG00000183632
Entrez:   24150
Chromosome Mapping:   16p13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.980000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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