PrimePCR™ Probe Assay: TMEM89, Human

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RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040948
Assay Design:   exonic
Chromosome Location:   3:48658896-48659061question
Amplicon Length:   136
Splice Variants Targeted:   ENST00000239906 ENST00000574557 ENST00000547047 ENST00000271064 ENST00000457433 ENST00000551460 ENST00000267890 ENST00000243077 ENST00000548433 ENST00000546487 ENST00000551204 ENST00000330862 ENST00000454499 ENST00000548112 ENST00000301067 ENST00000560371 ENST00000339284 ENST00000393955 ENST00000393958 ENST00000393960 ENST00000409699 ENST00000422354 ENST00000395614 ENST00000360698 ENST00000377842 ENST00000548827 ENST00000399609 ENST00000256255 ENST00000521265 ENST00000523127 ENST00000545648 ENST

Gene Information

Description Not Available

Gene Symbol:   TMEM89
Gene Name:   transmembrane protein 89
Aliases:   Not Available
RefSeq:   NC_000003.11 NT_022517.18
Ensembl:   ENSG00000183396
Entrez:   440955
UniGene:   Hs.631922
Chromosome Mapping:   3p21.31

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 36.320000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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