PrimePCR™ Probe Assay: FAM156A, Human

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RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040940
Assay Design:   exonic
Chromosome Location:   X:52977648-52977770question
Amplicon Length:   93
Splice Variants Targeted:   ENST00000452154 ENST00000430150 ENST00000452021 ENST00000412319 ENST00000416841 ENST00000509613 ENST00000356333 ENST00000316310 ENST00000330025 ENST00000512364 ENST00000414076 ENST00000438003 ENST00000411516 ENST00000503775 ENST00000375473 ENST00000451084 ENST00000447562 ENST00000510517 ENST00000593751 ENST00000596733

Gene Information

Description Not Available

Gene Symbol:   FAM156A
Gene Name:   family with sequence similarity 156, member A
Aliases:   DKFZp686B22211, PRO0659, TMEM29
RefSeq:   NC_000023.10 NT_011630.14
Ensembl:   ENSG00000182646
Entrez:   29057
UniGene:   Hs.6451
Chromosome Mapping:   Xp11.22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998900
y-intercept 35.910000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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