PrimePCR™ Probe Assay: GOLGA8J, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: GOLGA8J, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: GOLGA8J, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040928
Assay Design:   exonic
Chromosome Location:   15:30379778-30380967question
Amplicon Length:   104
Splice Variants Targeted:   ENST00000563027 ENST00000340249 ENST00000269143 ENST00000567927 ENST00000341650 ENST00000569052 ENST00000327271 ENST00000562783 ENST00000566740 ENST00000410013 ENST00000512626 ENST00000509311 ENST00000292928 ENST00000439428 ENST00000569659 ENST00000448387 ENST00000382856 ENST00000382852 ENST00000289921 ENST00000329517 ENST00000403325 ENST00000301727 ENST00000565090 ENST00000564139 ENST00000436636 ENST00000341852 ENST00000284483 ENST00000475336 ENST00000357327 ENST00000460047 ENST00000488470 ENST

Gene Information

Description Not Available

Gene Symbol:   GOLGA8J
Gene Name:   golgin A8 family, member J
Aliases:   Not Available
RefSeq:   NC_000015.9 NT_010194.17
Ensembl:   ENSG00000179938
Entrez:   653073
Chromosome Mapping:   15q13.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998300
y-intercept 36.020000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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