PrimePCR™ Probe Assay: ZSCAN2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: ZSCAN2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ZSCAN2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040918
Assay Design:   exonic
Chromosome Location:   15:85147126-85147237question
Amplicon Length:   82
Splice Variants Targeted:   ENST00000371191 ENST00000403491 ENST00000371185 ENST00000371187 ENST00000407417 ENST00000371189 ENST00000361677 ENST00000404826 ENST00000389531 ENST00000312541 ENST00000429381 ENST00000269919 ENST00000540936 ENST00000448803 ENST00000546275 ENST00000546148 ENST00000442073 ENST00000334141 ENST00000502939 ENST00000379358 ENST00000327179 ENST00000541040 ENST00000538076 ENST00000485222 ENST00000488625 ENST00000542879 ENST00000320985 ENST00000559858 ENST00000560721 ENST00000437844 ENST00000542815 ENST

Gene Information

The protein encoded by this gene contains several copies of zinc finger motif which is commonly found in transcriptional regulatory proteins. Studies in mice show that this gene is expressed during embryonic development and specifically in the testis in adult mice suggesting that it may play a role in regulating genes in germ cells. Alternative splicing of this gene results in several transcript variants encoding different isoforms. [provided by RefSeq Jul 2008]

Gene Symbol:   ZSCAN2
Gene Name:   zinc finger and SCAN domain containing 2
Aliases:   FLJ20595, ZFP29, ZNF854
RefSeq:   NC_000015.9 NT_010274.17
Ensembl:   ENSG00000176371
Entrez:   54993
Chromosome Mapping:   15q25.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 35.490000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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