PrimePCR™ Probe Assay: TOMM5, Human

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RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040914
Assay Design:   exonic
Chromosome Location:   9:37592452-37592568question
Amplicon Length:   87
Splice Variants Targeted:   ENST00000523826 ENST00000339803 ENST00000379808 ENST00000552788 ENST00000549814 ENST00000524140 ENST00000341319 ENST00000558729 ENST00000341618 ENST00000399935 ENST00000399934 ENST00000399947 ENST00000339024 ENST00000399928 ENST00000286791 ENST00000545939 ENST00000300576 ENST00000434739 ENST00000361937 ENST00000401393 ENST00000377773 ENST00000321301 ENST00000401811 ENST00000544379 ENST00000453097 ENST00000421285 ENST00000279058 ENST00000417257 ENST00000402883 ENST00000290438 ENST00000356884 ENST

Gene Information

Description Not Available

Gene Symbol:   TOMM5
Gene Name:   translocase of outer mitochondrial membrane 5 homolog (yeast)
Aliases:   C9orf105, RP11-263I4.1, Tom5, bA613M10.3
RefSeq:   NC_000009.11 NT_008413.18
Ensembl:   ENSG00000175768
Entrez:   401505
UniGene:   Hs.130774
Chromosome Mapping:   9p13.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 39.310000
Efficiency 86

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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