PrimePCR™ Probe Assay: AMY1B, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: AMY1B, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: AMY1B, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040908
Assay Design:   exonic
Chromosome Location:   1:104238187-104238917question
Amplicon Length:   102
Splice Variants Targeted:   ENST00000003084 ENST00000454343 ENST00000361355 ENST00000435302 ENST00000453959 ENST00000414303 ENST00000430659 ENST00000370083 ENST00000422549 ENST00000370080 ENST00000446703 ENST00000425410 ENST00000330330 ENST00000370079

Gene Information

Amylases are secreted proteins that hydrolyze 14-alpha-glucoside bonds in oligosaccharides and polysaccharides and thus catalyze the first step in digestion of dietary starch and glycogen. The human genome has a cluster of several amylase genes that are expressed at high levels in either salivary gland or pancreas. This gene encodes an amylase isoenzyme produced by the salivary gland. [provided by RefSeq Jul 2008]

Gene Symbol:   AMY1B
Gene Name:   amylase, alpha 1B (salivary)
Aliases:   AMY1, AMY1A, AMY1C, MGC177995
RefSeq:   NC_000001.10 NG_004750.1 NT_032977.9
Ensembl:   ENSG00000174876
Entrez:   277
Chromosome Mapping:   1p21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.930000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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