PrimePCR™ Probe Assay: TMEM223, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: TMEM223, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: TMEM223, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040867
Assay Design:   exonic
Chromosome Location:   11:62559203-62559348question
Amplicon Length:   116
Splice Variants Targeted:   ENST00000345506 ENST00000590949 ENST00000546010 ENST00000357503 ENST00000330884 ENST00000317961 ENST00000382806 ENST00000440077 ENST00000541639 ENST00000567657 ENST00000397637 ENST00000370033 ENST00000357277 ENST00000303843 ENST00000380064 ENST00000264703 ENST00000453654 ENST00000288490 ENST00000446122 ENST00000424189 ENST00000528367 ENST00000525631 ENST00000307366

Gene Information

Description Not Available

Gene Symbol:   TMEM223
Gene Name:   transmembrane protein 223
Aliases:   MGC3196
RefSeq:   NC_000011.9 NT_167190.1
Ensembl:   ENSG00000168569
Entrez:   79064
Chromosome Mapping:   11q12.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.490000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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