PrimePCR™ Probe Assay: ZNF3, Human

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RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040855
Assay Design:   exonic
Chromosome Location:   7:99674928-99675035question
Amplicon Length:   78
Splice Variants Targeted:   ENST00000164133 ENST00000527441 ENST00000424718 ENST00000548649 ENST00000413658 ENST00000299667 ENST00000449785 ENST00000428683 ENST00000415068 ENST00000292393 ENST00000424697 ENST00000303915 ENST00000518255 ENST00000440248 ENST00000550212 ENST00000356861 ENST00000450764 ENST00000589149 ENST00000592287 ENST00000588216 ENST00000425528 ENST00000441499 ENST00000383159 ENST00000549232 ENST00000424713 ENST00000549652 ENST00000262283 ENST00000451159 ENST00000553092 ENST00000412923 ENST00000344876 ENST

Gene Information

Description Not Available

Gene Symbol:   ZNF3
Gene Name:   zinc finger protein 3
Aliases:   A8-51, FLJ20216, HF.12, KOX25, PP838, Zfp113
RefSeq:   NC_000007.13 NT_007933.15
Ensembl:   ENSG00000166526
Entrez:   7551
UniGene:   Hs.435302
Chromosome Mapping:   7q22.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 35.860000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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