PrimePCR™ Probe Assay: ANKRD30BL, Human

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RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040839
Assay Design:   exonic
Chromosome Location:   2:132914550-132914901question
Amplicon Length:   159
Splice Variants Targeted:   ENST00000325874 ENST00000395123 ENST00000395124 ENST00000352187 ENST00000529554 ENST00000533524 ENST00000374810 ENST00000374809 ENST00000358984 ENST00000447268 ENST00000268711 ENST00000395757 ENST00000355815 ENST00000261646 ENST00000338854 ENST00000335647 ENST00000335643 ENST00000367411 ENST00000358196 ENST00000593535 ENST00000244869 ENST00000409867 ENST00000594133 ENST00000576555

Gene Information

Description Not Available

Gene Symbol:   ANKRD30BL
Gene Name:   ankyrin repeat domain 30B-like
Aliases:   ANKRD30BP3, NCRNA00164
RefSeq:   NC_000002.11 NT_022135.16
Ensembl:   ENSG00000163046
Entrez:   554226
UniGene:   Hs.380689
Chromosome Mapping:   2q21.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 36.320000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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