PrimePCR™ Probe Assay: CMPK1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: CMPK1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: CMPK1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040833
Assay Design:   exonic
Chromosome Location:   1:47799652-47799746question
Amplicon Length:   65
Splice Variants Targeted:   ENST00000546544 ENST00000538496 ENST00000341771 ENST00000372086 ENST00000372084 ENST00000292609 ENST00000371873 ENST00000471289 ENST00000450808 ENST00000360737 ENST00000393235 ENST00000464064 ENST00000358127 ENST00000332312 ENST00000409816 ENST00000409090 ENST00000389524 ENST00000428314 ENST00000407327 ENST00000335757 ENST00000586078 ENST00000330394 ENST00000355502 ENST00000396879 ENST00000329271

Gene Information

This gene encodes one of the enzymes required for cellular nucleic acid biosynthesis. This enzyme catalyzes the transfer of a phosphate group from ATP to CMP UMP or dCMP to form the corresponding diphosphate nucleotide. Alternate splicing results in both coding and non-coding transcript variants. [provided by RefSeq Feb 2012]

Gene Symbol:   CMPK1
Gene Name:   cytidine monophosphate (UMP-CMP) kinase 1, cytosolic
Aliases:   CMK, CMPK, UMK, UMP-CMPK, UMPK
RefSeq:   NC_000001.10 NT_032977.9
Ensembl:   ENSG00000162368
Entrez:   51727
Chromosome Mapping:   1p32

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.440000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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