PrimePCR™ Probe Assay: C11orf66, Human

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RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040832
Assay Design:   exonic
Chromosome Location:   11:61257982-61258302question
Amplicon Length:   61
Splice Variants Targeted:   ENST00000353836 ENST00000439889 ENST00000529627 ENST00000432469 ENST00000412770 ENST00000587283 ENST00000580098 ENST00000432063 ENST00000338608 ENST00000542951 ENST00000535545 ENST00000366212 ENST00000538185 ENST00000264436 ENST00000562520 ENST00000299366 ENST00000284382 ENST00000486996

Gene Information

Description Not Available

Gene Symbol:   C11orf66
Gene Name:   chromosome 11 open reading frame 66
Aliases:   FLJ32771, IIIG9
RefSeq:   NC_000011.9 NT_167190.1
Ensembl:   ENSG00000162148
Entrez:   220004
UniGene:   Not Available
Chromosome Mapping:   11q12.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.560000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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