PrimePCR™ Probe Assay: KCNMA1, Human

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Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040818
Assay Design:   exonic
Chromosome Location:   10:79397049-79397231question
Amplicon Length:   153
Splice Variants Targeted:   ENST00000244333 ENST00000595358 ENST00000323084 ENST00000397916 ENST00000496391 ENST00000397912 ENST00000354783 ENST00000372437 ENST00000372408 ENST00000457953 ENST00000372421 ENST00000404771 ENST00000372440 ENST00000372443 ENST00000372403 ENST00000286628 ENST00000286627 ENST00000406533 ENST00000354353 ENST00000404857 ENST00000564985 ENST00000569623

Gene Information

MaxiK channels are large conductance voltage and calcium-sensitive potassium channels which are fundamental to the control of smooth muscle tone and neuronal excitability. MaxiK channels can be formed by 2 subunits: the pore-forming alpha subunit which is the product of this gene and the modulatory beta subunit. Intracellular calcium regulates the physical association between the alpha and beta subunits. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq Jul 2008]

Gene Symbol:   KCNMA1
Gene Name:   potassium large conductance calcium-activated channel, subfamily M, alpha member 1
Aliases:   BKTM, DKFZp686K1437, KCa1.1, MGC71881, MaxiK, SAKCA, SLO, SLO-ALPHA, SLO1, bA205K10.1, mSLO1
RefSeq:   NC_000010.10 NG_012270.1 NT_030059.13
Ensembl:   ENSG00000156113
Entrez:   3778
UniGene:   Hs.144795
Chromosome Mapping:   10q22.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.270000
Efficiency 104

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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