PrimePCR™ Probe Assay: MZT2B, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: MZT2B, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: MZT2B, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040813
Assay Design:   exonic
Chromosome Location:   2:130940202-130940294question
Amplicon Length:   63
Splice Variants Targeted:   ENST00000539812 ENST00000287820 ENST00000397000 ENST00000397026 ENST00000397012 ENST00000397015 ENST00000309576 ENST00000397010 ENST00000307050 ENST00000382902 ENST00000452918 ENST00000601879 ENST00000600377 ENST00000314167 ENST00000511163 ENST00000370060 ENST00000370055 ENST00000361699 ENST00000543994 ENST00000370057 ENST00000538883 ENST00000361981 ENST00000216036 ENST00000406876 ENST00000528282 ENST00000530481 ENST00000445890 ENST00000337057 ENST00000392427 ENST00000368128 ENST00000527411 ENST

Gene Information

Description Not Available

Gene Symbol:   MZT2B
Gene Name:   mitotic spindle organizing protein 2B
Aliases:   FAM128B, FLJ14346, MGC87017, MOZART2B
RefSeq:   NC_000002.11 NT_022135.16
Ensembl:   ENSG00000152082
Entrez:   80097
Chromosome Mapping:   2q21.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998500
y-intercept 35.570000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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