PrimePCR™ Probe Assay: TPM3, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: TPM3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: TPM3, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040793
Assay Design:   exonic
Chromosome Location:   1:154142876-154143146question
Amplicon Length:   61
Splice Variants Targeted:   ENST00000392464 ENST00000392465 ENST00000361234 ENST00000319778 ENST00000375110 ENST00000263062 ENST00000566740 ENST00000265348 ENST00000535468 ENST00000344824 ENST00000300933 ENST00000592822 ENST00000538887 ENST00000374403 ENST00000374388 ENST00000166345 ENST00000513435 ENST00000435029 ENST00000378300 ENST00000378292 ENST00000329305 ENST00000360958 ENST00000368533 ENST00000330188 ENST00000341485 ENST00000341372 ENST00000328159 ENST00000271850 ENST00000302206 ENST00000368531 ENST00000323144 ENST

Gene Information

This gene encodes a member of the tropomyosin family of actin-binding proteins involved in the contractile system of striated and smooth muscles and the cytoskeleton of non-muscle cells. Tropomyosins are dimers of coiled-coil proteins that polymerize end-to-end along the major groove in most actin filaments. They provide stability to the filaments and regulate access of other actin-binding proteins. In muscle cells they regulate muscle contraction by controlling the binding of myosin heads to the actin filament. Mutations in this gene result in autosomal dominant nemaline myopathy and oncogenes formed by chromosomal translocations involving this locus are associated with cancer. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   TPM3
Gene Name:   tropomyosin 3
Aliases:   FLJ41118, MGC14582, MGC3261, MGC72094, NEM1, OK/SW-cl.5, TM-5, TM3, TM30, TM30nm, TPMsk3, TRK, hscp30
RefSeq:   NC_000001.10 NT_004487.19 NG_008621.1
Ensembl:   ENSG00000143549
Entrez:   7170
Chromosome Mapping:   1q21.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 35.290000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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