PrimePCR™ Probe Assay: CLEC18B, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: CLEC18B, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: CLEC18B, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040785
Assay Design:   exonic
Chromosome Location:   16:74442730-74442865question
Amplicon Length:   106
Splice Variants Targeted:   ENST00000393701 ENST00000568461 ENST00000288040 ENST00000449317 ENST00000541793 ENST00000314151 ENST00000569347 ENST00000536907 ENST00000370220 ENST00000352456 ENST00000595018 ENST00000392006 ENST00000378215 ENST00000602130 ENST00000593587 ENST00000263367 ENST00000599614 ENST00000409111 ENST00000260983 ENST00000586598 ENST00000339953 ENST00000308287

Gene Information

Description Not Available

Gene Symbol:   CLEC18B
Gene Name:   C-type lectin domain family 18, member B
Aliases:   MRCL2
RefSeq:   NC_000016.9 NT_010498.15
Ensembl:   ENSG00000140839
Entrez:   497190
Chromosome Mapping:   16q22.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998900
y-intercept 35.280000
Efficiency 104

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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