PrimePCR™ Probe Assay: RPLP1, Human


Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040779
Assay Design:   exonic
Chromosome Location:   15:69745258-69745987question
Amplicon Length:   73
Splice Variants Targeted:   ENST00000395353 ENST00000357402 ENST00000278937 ENST00000256151 ENST00000371922 ENST00000536433 ENST00000382839 ENST00000324666 ENST00000502458 ENST00000505311 ENST00000513350 ENST00000545951 ENST00000373018 ENST00000259748 ENST00000429989 ENST00000372164 ENST00000535456 ENST00000525089 ENST00000339772 ENST00000389881 ENST00000332904 ENST00000372117 ENST00000440770 ENST00000515425 ENST00000512049 ENST00000538184 ENST00000394358 ENST00000338316 ENST00000537121 ENST00000470649 ENST00000367161 ENST

Gene Information

Ribosomes the organelles that catalyze protein synthesis consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal phosphoprotein that is a component of the 60S subunit. The protein which is a functional equivalent of the E. coli L7/L12 ribosomal protein belongs to the L12P family of ribosomal proteins. It plays an important role in the elongation step of protein synthesis. Unlike most ribosomal proteins which are basic the encoded protein is acidic. Its C-terminal end is nearly identical to the C-terminal ends of the ribosomal phosphoproteins P0 and P2. The P1 protein can interact with P0 and P2 to form a pentameric complex consisting of P1 and P2 dimers and a P0 monomer. The protein is located in the cytoplasm. Two alternatively spliced transcript variants that encode different proteins have been observed. As is typical for genes encoding ribosomal proteins there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq Jul 2008]

Gene Symbol:   RPLP1
Gene Name:   ribosomal protein, large, P1
Aliases:   FLJ27448, LP1, MGC5215, P1, RPP1
RefSeq:   NC_000015.9 NT_010194.17
Ensembl:   ENSG00000137818
Entrez:   6176
UniGene:   Hs.356502
Chromosome Mapping:   15q22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 36.060000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download