PrimePCR™ Probe Assay: TAF1L, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: TAF1L, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: TAF1L, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040729
Assay Design:   exonic
Chromosome Location:   9:32634300-32634439question
Amplicon Length:   110
Splice Variants Targeted:   ENST00000242310 ENST00000373790 ENST00000276072 ENST00000449580 ENST00000423759

Gene Information

This locus is intronless and apparently arose in the primate lineage from retrotransposition of the transcript from the multi-exon TAF1 locus on the X chromosome. The gene is expressed in male germ cells and the product has been shown to function interchangeably with the TAF1 product. [provided by RefSeq Aug 2009]

Gene Symbol:   TAF1L
Gene Name:   TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 210kDa-like
Aliases:   MGC134910, TAF2A2
RefSeq:   NC_000009.11 NT_008413.18
Ensembl:   ENSG00000122728
Entrez:   138474
Chromosome Mapping:   9p21.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.880000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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