PrimePCR™ Probe Assay: OBP2A, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: OBP2A, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: OBP2A, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040726
Assay Design:   exonic
Chromosome Location:   9:138439024-138439736question
Amplicon Length:   60
Splice Variants Targeted:   ENST00000507384 ENST00000317447 ENST00000340780 ENST00000342114 ENST00000371776 ENST00000539850 ENST00000305249 ENST00000372034 ENST00000372032 ENST00000421745 ENST00000254799 ENST00000439371 ENST00000283415 ENST00000313382 ENST00000532801 ENST00000533259 ENST00000493130 ENST00000478649 ENST00000333834 ENST00000562137 ENST00000575495

Gene Information

This gene encodes a small extracellular protein belonging to the lipocalin superfamily. The protein is thought to transport small hydrophobic volatile molecules or odorants through the nasal mucus to olfactory receptors and may also function as a scavenger of highly concentrated or toxic odors. The protein is expressed as a monomer in the nasal mucus and can bind diverse types of odorants with a higher affinity for aldehydes and fatty acids. This gene and a highly similar family member are located in a cluster of lipocalin genes on chromosome 9. Alternatively spliced transcript variants have been described but their biological validity has not been determined. [provided by RefSeq Jul 2008]

Gene Symbol:   OBP2A
Gene Name:   odorant binding protein 2A
Aliases:   OBP, OBP2C, OBPIIa, hOBPIIa
RefSeq:   NC_000009.11 NT_019501.13
Ensembl:   ENSG00000122136
Entrez:   29991
Chromosome Mapping:   9q34

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998400
y-intercept 35.640000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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