PrimePCR™ Probe Assay: GPR31, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040720
Assay Design:   exonic
Chromosome Location:   6:167570683-167570772question
Amplicon Length:   60
Splice Variants Targeted:   ENST00000449592 ENST00000541467 ENST00000540377 ENST00000380833 ENST00000380829 ENST00000421085 ENST00000390649 ENST00000361050 ENST00000366834

Gene Information

Description Not Available

Gene Symbol:   GPR31
Gene Name:   G protein-coupled receptor 31
Aliases:   Not Available
RefSeq:   NC_000006.11 NT_025741.15
Ensembl:   ENSG00000120436
Entrez:   2853
Chromosome Mapping:   6q27

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.720000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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