PrimePCR™ Probe Assay: FAM178A, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: FAM178A, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: FAM178A, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040718
Assay Design:   exonic
Chromosome Location:   10:102672747-102672846question
Amplicon Length:   70
Splice Variants Targeted:   ENST00000331289 ENST00000407471 ENST00000435822 ENST00000371865 ENST00000367651 ENST00000398898 ENST00000417901 ENST00000453664 ENST00000398892 ENST00000398888 ENST00000434032 ENST00000306026 ENST00000491554 ENST00000371856 ENST00000360426 ENST00000347458 ENST00000340954 ENST00000371802 ENST00000371792 ENST00000371828 ENST00000370271 ENST00000238961 ENST00000370269 ENST00000272937 ENST00000409160 ENST00000409002 ENST00000291416 ENST00000424809 ENST00000550288 ENST00000329517 ENST00000404283 ENST

Gene Information

Description Not Available

Gene Symbol:   FAM178A
Gene Name:   family with sequence similarity 178, member A
Aliases:   C10orf6, MGC90157
RefSeq:   NC_000010.10 NT_030059.13
Ensembl:   ENSG00000119906
Entrez:   55719
Chromosome Mapping:   10q24.31

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997000
y-intercept 35.230000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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